The calculation for the influence of high-speed railway on knowledge spillover is based on the results of global instantaneous equilibrium in the mechanism explanation of knowledge spillover. In real production, the interaction between the high-speed railway and the regional innovation system is dynamic and local. In order to simulate the impact of high-speed railway on innovation activities in the time dimension, it is necessary to simulate scenarios under appropriate parameter assumptions. Based on the interaction of economic participants, a discrete evolutionary simulation model is established, which is helpful to predict and estimate the evolution of spatial effect of high-speed railway according to the theory of cellular automata. It is concluded that high-speed railway accelerates the formation of knowledge innovation industry cluster in the region in the process of regional knowledge innovation and evolution. Under the influence of high-speed railway, the node city will gradually evolve into a regional innovation center. By comparing the production evolution of knowledge innovation system with and without high-speed railway, the results show that high-speed railway has a more significant impact on knowledge spillover in higher knowledge privatization environment. Under the background of low labor migration rate, high-speed railway has increased the potential of regional innovation to external knowledge spillover. In the case of higher labor migration rate, the convergence rate of influence of high-speed railway on the concentration of innovation is faster.
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